Journal: Molecular Systems Biology

Article Title: Oncogenic PIK3CA corrupts growth factor signaling specificity

doi: 10.1038/s44320-024-00078-x

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Next, 75 µl of DPBS was added, followed by 19 µl of 5× fish skin gelatin blocking agent (Biotium #22010) diluted in PBS/T (PBS with 0.05% Tween-20) with 0.5% Triton X-100 (5X concentration, diluted to 0.1% once added to the DPBS).

Techniques: Knock-Out, Recombinant, Western Blot, Mass Cytometry, Sequencing, Clone Assay, Mutagenesis, Cell Culture, Membrane, Transfection, Lysis, Extraction, Protease Inhibitor, Sterility, Gel Extraction, Reverse Transcription, SYBR Green Assay, Blocking Assay, Staining, Software, Cytometry, Real-time Polymerase Chain Reaction, Microscopy

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: BBB score of each group after 7 and 14 days of treatment. * P < 0.05 versus 7d SCI; # P < 0.05 versus 14d SCI; ☆ P < 0.05 versus 7d EA; ▵ P < 0.05 versus 7d Y-27632; ☆ P < 0.05 versus 14d EA; ▴ P < 0.05 versus 14d Y-27632. Data are expressed as the mean ± SD (two-way analysis of variance and Student–Newman–Keuls post hoc test, n = 16 or 8 rats/group). sham: sham operation; SCI: SCI; EA: electroacupuncture; Y: blocking agent Y27632.

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Blocking Assay

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: Activity of cPLA2 and level of PGE2 following SCI. (A) Compiled results in a bar graph for the activity of cPLA2 at 7 and 14 days after treatment. (B) Compiled results in a bar graph for the level of PGE2 at 7 and 14 days after treatment. & P < 0.05 versus 7d sham; ^ P < 0.05 versus 14d sham; * P < 0.05 versus 7d SCI; # P < 0.05 versus 14d SCI; ☆ P < 0.05 versus 7d EA; △ P < 0.05 versus 7d Y-27632; ☆ P < 0.05 versus 14d EA; ▴ P < 0.05 versus 14d Y-27632. Data are shown as the mean ± standard error of the mean (two-way analysis of variance and Student–Newman–Keuls post hoc test, n = 8 rats/group). sham: sham operation; SCI: SCI; EA: electroacupuncture; Y: blocking agent Y27632.

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Activity Assay, Blocking Assay

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: Effect of EA on changes in mRNA and protein expression levels of RhoA and ROCKII of injured spinal cords. (A,B) Western blotting bands for RhoA, ROCKII and GAPDH in different groups at 7 and 14 days. (C) Compiled results in a bar graph for RhoA protein expression. (D) Compiled results in a bar graph for ROCKII protein expression. (E) Compiled results in a bar graph for RhoA mRNA expression. (F) Compiled results in a bar graph for ROCKII mRNA expression. Y-axis indicates the relative grayscale value of protein bands (the ratio of target grayscale values to loading control grayscale values). & P < 0.05 versus 7d sham; ^ P < 0.05 versus 14d sham; * P < 0.05 versus 7d SCI; # P < 0.05 versus 14d SCI; ☆ P < 0.05 versus 7d EA; △ P < 0.05 versus 7d Y-27632; ☆ P < 0.05 versus 14d EA; ▴ P < 0.05 versus 14d Y-27632. Data are expressed as the mean ± SD (two-way analysis of variance and Student–Newman–Keuls post hoc test, n = 4 rats/group). sham: sham operation; SCI:SCI; EA:electroacupuncture; Y:blocking agent Y27632. ROCKII: RhoAssociated kinase II. GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Expressing, Western Blot, Blocking Assay

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: mRNA expression of RhoA and ROCKII in injured spinal cords at 7 and 14 days. In situ hybridization positive cells contain brown spot or particles as indicated by the arrows. sham: sham operation; SCI: SCI; EA: electroacupuncture; Y: blocking agent Y27632; ROCK II: Rho-associated kinase II. (A) mRNA expression of Rho-A at 7 days; (B) mRNA expression of RhoA at 14 days; (C) mRNA expression of ROCKII at 7 days; (D) mRNA expression of ROCKII at 14 days. (n = 4 rats/group).

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Expressing, In Situ Hybridization, Blocking Assay

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: HE and Nissl staining in each group. Neuron cell was indicated by the black arrows, and neuroglia cell was indicated by the white arrows in HE staining and by the red arrows in Nissl staining. (A,B) HE staining at 7 days; (C,D): HE staining at 14 days; (E) Nissl staining at 7 days; (F) Nissl staining at 14 days. (n = 4 rats/group) sham: sham operation; SCI: SCI; EA: electroacupuncture; Y: blocking agent Y27632.

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Staining, Blocking Assay

Journal: The Journal of Spinal Cord Medicine

Article Title: The mechanism of electroacupuncture for treating spinal cord injury rats by mediating Rho/Rho-associated kinase signaling pathway

doi: 10.1080/10790268.2019.1665612

Figure Lengend Snippet: Phosphorylated myosin light chain(p-MLC) and MLC activation following SCI. (A,B) Western blotting bands for p-MLC, MLC and GAPDH in different groups at 7 and 14 days. (C) Compiled results in a bar graph for the ratio of p-MLC/GAPDH expression. (D) Compiled results in a bar graph for the ratio of MLC/GAPDH expression. (E) Compiled results in a bar graph for the ratio of p-MLC/MLC. & P < 0.05 versus 7d sham; ^ P < 0.05 versus 14d sham; * P < 0.05 versus 7d SCI; # P < 0.05 versus 14d SCI; ☆ P < 0.05 versus 7d EA; ☆ P < 0.05 versus 14d EA. Data are shown as the mean ± standard error of the mean (two-way analysis of variance and Student–Newman–Keuls post hoc test, n = 4 rats/group). sham: sham operation; SCI: SCI; EA: electroacupuncture; Y: blocking agent Y27632. MLC: myosin light chain. p-MLC: phosphorylated MLC. GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Rats in the Y27632 treatment group received the blocking agent Y27632 (Qilu Pharmaceutical Co., Ltd., Jinan, Shandong Province, China) by subdural injection via a microinjector from a homemade PE10 tube (the 18 μg, 18 μg Y27632 lyophilized powders were dissolved in 30 μL phosphate buffered saline solution; each rat received 0.54 mL/kg), once per day, lasting for 14 days.8.

Techniques: Activation Assay, Western Blot, Expressing, Blocking Assay